A Role for Taok2 in Listeria monocytogenes Vacuolar Escape. (25th June 2020)

• Record Type: Journal Article
• Authors: Quereda, Juan J; Morel, Camille; Lopez-Montero, Noelia; Ziveri, Jason; Rolland, Steven; Grenier, Théodore; Aulner, Nathalie; Danckaert, Anne; Charbit, Alain; Enninga, Jost; Cossart, Pascale; Pizarro-Cerdá, Javier
• Is Part Of: Journal of infectious diseases. Volume 225:Number 6(2022)
• Page Start: 1005
• Page End: 1010
• Publication Date: 2020-06-25
• Subjects: Listeria monocytogenes -- Taok2 -- STE1-like kinase -- vacuolar escape -- siRNA screen; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiaa367
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.