Macluraxanthone B inhibits LPS-induced inflammatory responses in RAW264.7 and BV2 cells by regulating the NF-κB and MAPK signaling pathways. (2nd January 2022)

• Record Type: Journal Article
• Authors: Ko, Wonmin; Kim, Kwan-Woo; Liu, Zhiming; Dong, Linsha; Yoon, Chi-Su; Lee, Hwan; Kim, Youn-Chul; Oh, Hyuncheol; Lee, Dong-Sung; Kim, Sam Cheol
• Is Part Of: Immunopharmacology and immunotoxicology. Volume 44:Number 1(2022)
• Page Start: 67
• Page End: 75
• Publication Date: 2022-01-02
• Subjects: Macluraxanthone B -- nuclear factor kappa B -- mitogen-activated protein kinase -- heme oxygenase-1 -- anti-inflammation; Immunopharmacology -- Periodicals; Immunotoxicology -- Periodicals; Antibody-toxin conjugates -- Periodicals; Immunology -- Periodicals; 615.37
• DOI: 10.1080/08923973.2021.2006215
• Languages: English
• ISSNs: 0892-3973

Abstract

Abstract: Objective: The prenylated xanthones compounds, macluraxanthone B (MCXB) was isolated from the MeOH extracts of Cudrania tricuspidata . In this study, we investigated the effect of MCXB on inflammatory response. Materials and Methods: Anti-inflammatory effects of MCXB were examined in lipopolysaccharide (LPS)-stimulated RAW264.7 and BV2 cells. We observed their anti-inflammatory effects by ELISA, western blot analysis, and immunofluorescence. Results: MCXB significantly inhibited the LPS-stimulated production of nitric oxide (NO), prostaglandin E2 (PGE2 ), interleukin-6 (IL-6), and tumor necrosis factor (TNF)-α in RAW264.7 and BV2 cells. MCXB also reduced the LPS-induced expression of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 proteins. Incubating cells with MCXB prevented subsequent activation of the nuclear factor kappa B (NF-κB) signaling pathway by inhibiting the nuclear localization and DNA-binding activity of the p65 subunit induced by LPS. MCXB inhibited the phosphorylation of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38 mitogen-activated protein kinases (MAPKs) in RAW264.7 and BV2 cells. MCXB induced the expression of heme oxygenase (HO)-1 protein, and the inhibitory effect of MCXB on nitric oxide production was partially reversed by a selective HO-1 inhibitor. Discussion and conclusions: Our results suggested that the anti-inflammatory effect of MCXB is partly regulated by HO-1 induction. In conclusion, MCXB could be a useful candidate for the development of therapeutic and preventive agents to treat inflammatory diseases.