Cell-penetrating poly(disulfide)-based nanoquenchers (qCPDs) for self-monitoring of intracellular gene delivery. Issue 11 (18th January 2022)

• Record Type: Journal Article
• Authors: Lang, Wenjie; Liew, Si Si; Wang, Shuyi; Hong, Dawei; Zhu, Liquan; Du, Shubo; Jiang, Linye; Yao, Shao Q.; Ge, Jingyan
• Is Part Of: Chemical communications. Volume 58:Issue 11(2022)
• Page Start: 1792
• Page End: 1795
• Publication Date: 2022-01-18
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d1cc07020g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Cell-penetrating poly(disulfide)-based nanoquenchers ( q CPDs) were developed for both gene delivery and detection of gene release. Abstract : Monitoring gene delivery has significant benefits in gene therapy. Herein, we report a nanoquencher system by doping a FRET pair during nucleic acid-assisted cell penetrating poly(disulfide) (CPD) formation. Our results show that this strategy not only produces an efficient gene delivery polymer with minimal endolysosomal trapping, but also enables monitoring the release of the gene from the vehicle in live cells. This study further expanded the application of CPDs as promising tools in gene delivery.