SERS tags derived from silver nanoparticles and aryl diazonium salts for cell Raman imaging. Issue 4 (12th January 2022)

• Record Type: Journal Article
• Authors: Li, Da; Nizard, Philippe; Onidas, Delphine; Lamouri, Aazdine; Pinson, Jean; Mahouche-Chergui, Samia; Aubertin, Kelly; Gazeau, Florence; Luo, Yun; Mangeney, Claire
• Is Part Of: Nanoscale. Volume 14:Issue 4(2022)
• Page Start: 1452
• Page End: 1458
• Publication Date: 2022-01-12
• Subjects: Nanoscience -- Periodicals; Nanotechnology -- Periodicals; 620.505
• DOI: 10.1039/d1nr03148a
• Languages: English
• ISSNs: 2040-3364

Abstract

Abstract : Surface functionalization of silver nanoparticles by Raman reporters derived from aryl diazonium salts offers new opportunities for the design of Surface-Enhanced Raman Spectroscopy (SERS) labels for Cell Raman Imaging Abstract : The surface functionalization of silver nanoparticles (NPs) by Raman reporters has stimulated a wide interest in recent years for the design of Surface-Enhanced Raman Spectroscopy (SERS) labels. However, silver NPs are prone to oxidation and aggregation, which strongly limits their applications. The design of stable SERS tags based on Ag NPs still represents a major challenge for Raman bioimaging. We address this issue herein by taking advantage of aryl diazonium salt chemistry to obtain stable Ag NPs functionalized by multifunctional polyaryl layers bearing different Raman reporters (–NO2, –CN, –CCH). The resulting SERS-encoded Ag NPs were characterized by UV-vis absorption, transmission electron microscopy (TEM) and SERS. The formation of multilayers at the surface of Ag NPs gives access to new spectrally distinguishable SERS codes thus broadening the library of available Raman tags. Proof-of-concept Raman imaging experiments were performed on cancer cells (HeLa) after NP uptake, highlighting the large potentials of diazonium salt chemistry to design Ag NPs-based SERS labels for Raman bioimaging.