IDO1 Maintains Pluripotency of Primed Human Embryonic Stem Cells by Promoting Glycolysis. (10th June 2019)

• Record Type: Journal Article
• Authors: Liu, Xin; Wang, Meiyan; Jiang, Tao; He, Jingjin; Fu, Xuemei; Xu, Yang
• Is Part Of: Stem cells. Volume 37:Number 9(2019)
• Page Start: 1158
• Page End: 1165
• Publication Date: 2019-06-10
• Subjects: Human embryonic stem cells -- Pluripotency -- Differentiation -- Glycolysis -- Oxidative phosphorylation; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.3044
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: Human embryonic stem cells (hESCs) depend on glycolysis for energy supply and pluripotency and switch to oxidative phosphorylation upon differentiation. The underlying mechanisms remain unclear. Here, we demonstrate that indoleamine 2, 3-dioxygenase 1 (IDO1) is expressed in primed hESCs and its expression rapidly downregulated upon hESC differentiation. IDO1 is required to maintain pluripotency by suppressing mitochondria activity and promoting glycolysis through the increase of NAD + /NADH ratio. The upregulation of IDO1 during hESC differentiation suppresses the differentiation of hESCs into certain lineages of cells such as cardiomyocytes, which depend on oxidative phosphorylation to satisfy their high energy demand. Therefore, IDO1 plays important roles in maintaining the pluripotency of hESCs. Stem Cells 2019;37:1158–1165 : Abstract : Indoleamine 2, 3-dioxygenase 1 maintains pluripotency by increasing NAD + levels in primed human embryonic stem cells to promote metabolic switch from oxidative phosphorylation to glycolysis.