Multicentre appraisal of amyotrophic lateral sclerosis biofluid biomarkers shows primacy of blood neurofilament light chain. Issue 1 (9th February 2022)

• Record Type: Journal Article
• Authors: Thompson, Alexander G.; Gray, Elizabeth; Verber, Nick; Bobeva, Yoana; Lombardi, Vittoria; Shepheard, Stephanie R.; Yildiz, Ozlem; Feneberg, Emily; Farrimond, Lucy; Dharmadasa, Thanuja; Gray, Pamela; Edmond, Evan C.; Scaber, Jakub; Gagliardi, Delia; Kirby, Janine; Jenkins, Thomas M.; Fratta, Pietro; McDermott, Christopher J.; Manohar, Sanjay G.; Talbot, Kevin; Malaspina, Andrea; Shaw, Pamela J.; Turner, Martin R.
• Is Part Of: Brain communications. Volume 4:Issue 1(2022)
• Publication Date: 2022-02-09
• Subjects: amyotrophic lateral sclerosis -- motor neuron disease -- biomarker -- trial -- neurofilament; 616
• DOI: 10.1093/braincomms/fcac029
• Languages: English
• ISSNs: 2632-1297

Abstract

Abstract: The routine clinical integration of individualized objective markers of disease activity in those diagnosed with the neurodegenerative disorder amyotrophic lateral sclerosis is a key requirement for therapeutic development. A large, multicentre, clinic-based, longitudinal cohort was used to systematically appraise the leading candidate biofluid biomarkers in the stratification and potential therapeutic assessment of those with amyotrophic lateral sclerosis. Incident patients diagnosed with amyotrophic lateral sclerosis ( n = 258), other neurological diseases ( n = 80) and healthy control participants ( n = 101), were recruited and followed at intervals of 3–6 months for up to 30 months. Cerebrospinal fluid neurofilament light chain and chitotriosidase 1 and blood neurofilament light chain, creatine kinase, ferritin, complement C3 and C4 and C-reactive protein were measured. Blood neurofilament light chain, creatine kinase, serum ferritin, C3 and cerebrospinal fluid neurofilament light chain and chitotriosidase 1 were all significantly elevated in amyotrophic lateral sclerosis patients. First-visit plasma neurofilament light chain level was additionally strongly associated with survival (hazard ratio for one standard deviation increase in log10 plasma neurofilament light chain 2.99, 95% confidence interval 1.65–5.41, P = 0.016) and rate of disability progression, independent of other prognostic factors. A small increase in level was noted within the first 12 months after reported symptom onset (slope 0.031 log10 units per month, 95% confidence interval 0.012–0.049, P = 0.006). Modelling the inclusion of plasma neurofilament light chain as a therapeutic trial outcome measure demonstrated that a significant reduction in sample size and earlier detection of disease-slowing is possible, compared with using the revised Amyotrophic Lateral Sclerosis Functional Rating Scale. This study provides strong evidence that blood neurofilament light chain levels outperform conventional measures of disease activity at the group level. The application of blood neurofilament light chain has the potential to radically reduce the duration and cost of therapeutic trials. It might also offer a first step towards the goal of more personalized objective disease activity monitoring for those living with amyotrophic lateral sclerosis. Abstract : In a large, multicentre study of multiple measures, Thompson and Gray et al. demonstrate plasma neurofilament light chain as the leading prognostic biomarker in amyotrophic lateral sclerosis. Using clinical trial modelling, they show its potential as a sensitive outcome measure to more rapidly assess candidate therapeutics. Graphical Abstract: Graphical Abstract