3D hierarchically porous magnetic molybdenum trioxide@gold nanospheres as a nanogap-enhanced Raman scattering biosensor for SARS-CoV-2. Issue 3 (12th January 2022)

• Record Type: Journal Article
• Authors: Achadu, Ojodomo J.; Nwaji, Njemuwa; Lee, Dongkyu; Lee, Jaebeom; Akinoglu, Eser M.; Giersig, Michael; Park, Enoch Y.
• Is Part Of: Nanoscale advances. Volume 4:Issue 3(2022)
• Page Start: 871
• Page End: 883
• Publication Date: 2022-01-12
• Subjects: 620.5
• DOI: 10.1039/d1na00746g
• Languages: English
• ISSNs: 2516-0230

Abstract

Abstract : A multifunctional 3D magnetic molybdenum trioxide@gold nanosphere is deployed as a plug and play biosensor for SARS-CoV-2 spike proteins via ACE2-mediated immunocapture and magnetic-induced nanogap-enhanced Raman scattering (MINERS). Abstract : The global pandemic of COVID-19 is an example of how quickly a disease-causing virus can take root and threaten our civilization. Nowadays, ultrasensitive and rapid detection of contagious pathogens is in high demand. Here, we present a novel hierarchically porous 3-dimensional magnetic molybdenum trioxide–polydopamine-gold functionalized nanosphere (3D mag-MoO3 –PDA@Au NS) composed of plasmonic, semiconductor, and magnetic nanoparticles as a multifunctional nanosculptured hybrid. Based on the synthesized 3D mag-MoO3 –PDA@Au NS, a universal "plug and play" biosensor for pathogens is proposed. Specifically, a magnetically-induced nanogap-enhanced Raman scattering (MINERS) detection platform was developed using the 3D nanostructure. Through a magnetic actuation process, the MINERS system overcomes Raman signal stability and reproducibility challenges for the ultrasensitive detection of SARS-CoV-2 spike protein over a wide dynamic range up to a detection limit of 10 −15 g mL −1 . The proposed MINERS platform will facilitate the broader use of Raman spectroscopy as a powerful analytical detection tool in diverse fields.