Brief Report: Cell Cycle Dynamics of Human Pluripotent Stem Cells Primed for Differentiation. (10th June 2019)

• Record Type: Journal Article
• Authors: Shcherbina, Anna; Li, Jingling; Narayanan, Cyndhavi; Greenleaf, William; Kundaje, Anshul; Chetty, Sundari
• Is Part Of: Stem cells. Volume 37:Number 9(2019)
• Page Start: 1151
• Page End: 1157
• Publication Date: 2019-06-10
• Subjects: Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.3041
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator system adapted into hPSCs and perform RNA sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs toward differentiation. Stem Cells 2019;37:1151–1157 : Abstract : Identifying regulators of human pluripotent stem cell differentiation using Fluorescence Ubiquitin Cell Cycle Indicator technology. Schematic representation of the Fluorescence Ubiquitin Cell Cycle Indicator technology labeling individual late G1 phase nuclei in red and S/G2/M phase nuclei in green, whereas early G1 phase nuclei are double negative. Study design using H9-Fluorescence Ubiquitin Cell Cycle Indicator human pluripotent stem cells treated with or without 2% dimethylsulfoxide for 24 hours followed by cell cycle sorting and high throughput RNA-sequencing to identify regulators of proliferation and differentiation. Scale bar: 35 μm.