Telomerase expression marks transitional growth-associated skeletal progenitor/stem cells. (13th January 2021)

• Record Type: Journal Article
• Authors: Carlone, Diana L.; Riba-Wolman, Rebecca D.; Deary, Luke T.; Tovaglieri, Alessio; Jiang, Lijie; Ambruzs, Dana M.; Mead, Benjamin E.; Shah, Manasvi S.; Lengner, Christopher J.; Jaenisch, Rudolf; Breault, David T.
• Is Part Of: Stem cells. Volume 39:Number 3(2021)
• Page Start: 296
• Page End: 305
• Publication Date: 2021-01-13
• Subjects: mTert -- pluripotent -- postnatal bone growth -- skeletal stem cells -- telomerase; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.3318
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: Skeletal progenitor/stem cells (SSCs) play a critical role in postnatal bone growth and maintenance. Telomerase ( Tert ) activity prevents cellular senescence and is required for maintenance of stem cells in self-renewing tissues. Here we investigated the role of mTert -expressing cells in postnatal mouse long bone and found that mTert expression is enriched at the time of adolescent bone growth. mTert -GFP + cells were identified in regions known to house SSCs, including the metaphyseal stroma, growth plate, and the bone marrow. We also show that mTert -expressing cells are a distinct SSC population with enriched colony-forming capacity and contribute to multiple mesenchymal lineages, in vitro. In contrast, in vivo lineage-tracing studies identified mTert + cells as osteochondral progenitors and contribute to the bone-forming cell pool during endochondral bone growth with a subset persisting into adulthood. Taken together, our results show that mTert expression is temporally regulated and marks SSCs during a discrete phase of transitional growth between rapid bone growth and maintenance. Abstract : In long bones, osteochondral progenitor cells function during rapid bone growth while osteoadipogenic progenitors act during bone maintenance. We showed that mTert is expressed in an age-dependent manner and marks growth-associated osteochondral progenitor cells during transitional growth, a discrete time period between rapid bone growth and bone maintenance.