A Selective Cell Population from Dermis Strengthens Bone Regeneration. (8th August 2016)

• Record Type: Journal Article
• Authors: Wang, Tingliang; He, Jinguang; Zhang, Yang; Shi, Wenjun; Dong, Jiasheng; Pei, Ming; Zhu, Lian
• Is Part Of: Stem cells translational medicine. Volume 6:Number 1(2017)
• Page Start: 306
• Page End: 315
• Publication Date: 2016-08-08
• Subjects: Skin -- Bone morphogenetic protein receptor type IB -- Bone tissue engineering -- Coral -- Calvarial defect; Stem cells -- Periodicals; Regenerative medicine -- Periodicals; Periodicals; 616.0277405
• DOI: 10.5966/sctm.2015-0426
• Languages: English
• ISSNs: 2157-6564

Abstract

Abstract: Finding appropriate seed cells for bone tissue engineering remains a significant challenge. Considering that skin is the largest organ, we hypothesized that human bone morphogenetic protein receptor type IB (BmprIB)+ dermal cells could have enhanced osteogenic capacity in the healing of critical-sized calvarial defects in an immunodeficient mouse model. In this study, immunohistochemical staining revealed that BmprIB was expressed throughout reticular dermal cells; the positive expression rate of BmprIB was 3.5% ± 0.4% in freshly separated dermal cells, by flow cytometry. Furthermore, in vitro osteogenic capacity of BmprIB+ cells was confirmed by osteogenic-related staining and marker gene expression compared with unsorted dermal cells. In vivo osteogenic capacity was demonstrated by implantation of human BmprIB+ cell/coral constructs in the treatment of 4-mm diameter calvarial defects in an immunodeficient mouse model compared with implantation of unsorted cell/coral constructs and coral scaffold alone. These results indicate that the selective cell population BmprIB from human dermis is a promising osteogenic progenitor cell that can be a large-quantity and high-quality cell source for bone tissue engineering and regeneration.