Glycoenzyme Tool Development: Principles, Screening Methods, and Recent Advances†. Issue 6 (19th January 2022)

• Record Type: Journal Article
• Authors: Cui, Tongxiao; Man, Yi; Wang, Feifei; Bi, Shuyang; Lin, Liang; Xie, Ran
• Is Part Of: Chinese journal of chemistry. Volume 40:Issue 6(2022)
• Page Start: 746
• Page End: 758
• Publication Date: 2022-01-19
• Subjects: Carbohydrates -- Rational design -- Directed evolution -- High‐throughput screening -- Glycan detection method; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cjoc.202100770
• Languages: English
• ISSNs: 1001-604X

Abstract

Comprehensive Summary: To elucidate the relationship between carbohydrate structure and its biological function, glycoenzyme tools are widely applied in a variety of physiological and pathological scenarios. Intricate adjustment via glycoenzyme engineering improved the catalytic efficiency, extended the repertoire of accessible catalytic reactions, and tailored novel substrate specificities. In this review, we introduce the principles of glycoenzyme engineering and summarize the recent advances in the rational design and directed evolution of these enzymes, with an emphasis on screening methods in directed evolution. We also excerpt novel detection methods in glycobiology that can be adapted for the development of next‐generation glycoenzyme tools. Abstract : To understand fundamental glycobiology as well as compelling industrial or pharmaceutical applications, researchers have begun to develop novel glycoenzyme tools to manipulate glycosylation processes over the last decades. Herein, we describe the principles (rational mutagenesis and directed evolution tactics) and the screening methods of glycoenzyme engineering, with an emphasis toward recent advances for tailoring glycoenzyme activities.