Brief Report: Single-Cell Analysis Reveals Cell Division-Independent Emergence of Megakaryocytes From Phenotypic Hematopoietic Stem Cells. (6th August 2015)

• Record Type: Journal Article
• Authors: Roch, Aline; Trachsel, Vincent; Lutolf, Matthias P.
• Is Part Of: Stem cells. Volume 33:Number 10(2015:Oct.)
• Page Start: 3152
• Page End: 3157
• Publication Date: 2015-08-06
• Subjects: Adult hematopoietic stem cells -- Differentiation -- Hematopoiesis -- Megakaryocyte -- Stem cell plasticity; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.2106
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: Despite increasingly stringent methods to isolate hematopoietic stem cells (HSCs), considerable heterogeneity remains in terms of their long-term self-renewal and differentiation potential. Recently, the existence of long-lived, self-renewing, myeloid-restricted progenitors in the phenotypically defined HSC compartment has been revealed, but these cells remain poorly characterized. Here, we used an in vitro single-cell analysis approach to track the fate of 330 long-term HSCs (LT-HSC; Lin− cKit+ Sca-1+ CD150+ CD48− CD34−) cultured for 5 days under serum-free basal conditions. Our analysis revealed a highly heterogeneous behavior with approximately 15% of all phenotypic LT-HSCs giving rise to megakaryocytes (Mk). Surprisingly, in 65% of these cases, Mk development occurred in the absence of cell division. This observation suggests that myeloid-restricted progenitors may not derive directly from LT-HSCs but instead could share an identical cell surface marker repertoire. Stem Cells 2015;33:3152–3157