Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps. (15th January 2022)

• Record Type: Journal Article
• Authors: Cheng, Ke-Jia; Zhou, Min-Li; Liu, Yong-Cai; Zhou, Shui-Hong
• Other Names: Lor Nicola I. Academic Editor.
• Is Part Of: Mediators of inflammation. Volume 2022(2022)
• Publication Date: 2022-01-15
• Subjects: Inflammation -- Mediators -- Periodicals; Biological response modifiers -- Periodicals; Inflammation (Pathologie) -- Médiateurs; Immunomodulateurs; Biological response modifiers; Inflammation -- Mediators; Immunology; Autacoids; Immunologic Factors; Cell Adhesion Molecules; Cell Communication; Cytokines; Inflammation; Periodicals; Electronic journals; 616.0473
• DOI: 10.1155/2022/8609590
• Languages: English
• ISSNs: 0962-9351

Abstract

Abstract : Background . The Chinese subtype of CRSwNP may have a unique pathogenesis. This study was designed to seek the role of the PI3K/Akt/HIF-1 α pathway and IL-17A in CRSwNP. Methods . The total IgE, ECP, and IL-17A levels were determined by UniCAP100 and ELISA. The activity of MPO was detected by the biochemical techniques. The protein expressions of HIF-1 α, p-Akt, and PI3K were detected by the WB method. HIF-1 α and IL-17A mRNA levels were measured by RT-PCR. Results . The CRSwNP group showed significantly elevated MPO activity, PI3K, p-AKT protein, HIF-1 α, and IL-17A mRNA levels in nasal polyps. Stimulated by the TNF- α, the PI3K, p-AKT, HIF-1 α, and IL-17A levels significantly elevated in the fibroblasts. Inhibited by the Wortmannin, those indicators significantly declined in the fibroblasts. Conclusion . The PI3K/Akt/HIF-1 α pathway played a role in the pathogenesis of CRSwNP. The elevated IL-17A level might be responsible for the neutrophilic inflammation in CRSwNP. The PI3K/Akt/HIF-1 α pathway might regulate the IL-17A-related inflammation in CRSwNP.