Bxb1 phage recombinase assists genome engineering in Drosophila melanogaster. (January 2017)

• Record Type: Journal Article
• Authors: Voutev, Roumen; Mann, Richard S.
• Is Part Of: Biotechniques. Volume 62:Number 1(2017)
• Page Start: 37
• Page End: 38
• Publication Date: 2017-01
• Subjects: Bxb1 recombinase -- cassette exchange -- genome editing -- Drosophila melanogaster; Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114494
• Languages: English
• ISSNs: 0736-6205

Abstract

Rapid and reliable genome modifications provide the basis for detailed in vivo functional analysis of any genomic entity (gene, regulatory DNA, non-coding RNA, etc). With the advent of CRISPR/Cas9 genome editing technology, manipulation of a particular genomic locus has become a routine undertaking in variety of model organisms, including the fruit fly Drosophila melanogaster . To further diversify the available tools for genome engineering, we successfully harnessed the phage recombinase Bxb1 to perform recombinase-mediated cassette exchange (RMCE) in D. melanogaster . We demonstrate that Bxb1 possesses highly efficient recombinase activity and could be used alone or in conjunction with other currently available recombinases for creating platforms for cassette exchange of targeted loci.