Optimizing neural stem cell transfection with NeuroMag. (January 2017)

• Record Type: Journal Article
• Authors: Sapet, Cédric; Laurent, Nicolas; de Chevigny, Antoine; Gourrierec, Loïc Le; Bertosio, Elodie; Zelphati, Olivier; Béclin, Christophe
• Is Part Of: Biotechniques. Volume 62:Number 1(2017)
• Page Start: xii
• Publication Date: 2017-01
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114500
• Languages: English
• ISSNs: 0736-6205

Abstract

Protocol Summary: Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.