Protocol for intelligent high-content screening of zebrafish embryos on a standard widefield screening microscope. (January 2017)

• Record Type: Journal Article
• Authors: Peravali, Ravindra; Gehrig, Jochen; Giselbrecht, Stefan; Lütjohann, Dominic; Hadzhiev, Yavor; Müller, Ferenc; Liebel, Urban
• Is Part Of: Biotechniques. Volume 62:Number 1(2017)
• Page Start: xx
• Publication Date: 2017-01
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/000114506
• Languages: English
• ISSNs: 0736-6205

Abstract

Protocol Summary: Here we demonstrate a toolset for automated intelligent high-content screening of whole zebrafish embryos at cellular resolution on a standard wide-field screening microscope. Using custom-developed algorithms, predefined regions of interest, such as the brain, are automatically detected. The regions of interest are subsequently imaged automatically at high magnification, enabling rapid capture of cellular resolution data. standardized imaging of internal organs and cellular structures.