Time-resolved X-ray scattering studies of proteins. (October 2021)

• Record Type: Journal Article
• Authors: Cho, Hyun Sun; Schotte, Friedrich; Stadnytskyi, Valentyn; Anfinrud, Philip
• Is Part Of: Current opinion in structural biology. Volume 70(2021)
• Page Start: 99
• Page End: 107
• Publication Date: 2021-10
• Subjects: Time-resolved -- SAXS -- WAXS -- Protein structure dynamics; Molecular biology -- Periodicals; 570
• DOI: 10.1016/j.sbi.2021.05.002
• Languages: English
• ISSNs: 0959-440X

Abstract

Abstract: Time-resolved small- and wide-angle X-ray scattering studies of proteins in solution based on the pump-probe approach unveil structural information from intermediates over a broad range of length and time scales. In spite of the promise of this methodology, only a fraction of the wealth of information encoded in scattering data has been extracted in studies performed thus far. Here, we discuss the methodology, summarize results from recent time-resolved X-ray scattering studies, and examine the potential to extract additional information from these scattering curves.