Effects of micronutrients on the detection of extracellular superoxide produced by the harmful raphidophyte Chattonella antiqua in culture. (24th December 2021)

• Record Type: Journal Article
• Authors: Yamasaki, Yasuhiro; Hirayama, Ryunosuke; Yamamoto, Akiho; Yuasa, Koki; Shikata, Tomoyuki
• Editors: Campbell, Lisa
• Is Part Of: Journal of plankton research. Volume 44:Number 1(2022)
• Page Start: 36
• Page End: 47
• Publication Date: 2021-12-24
• Subjects: Chattonella -- fish mortality -- harmful algal bloom -- manganese -- reactive oxygen species -- superoxide; Plankton -- Periodicals; 578.776
• DOI: 10.1093/plankt/fbab087
• Languages: English
• ISSNs: 0142-7873

Abstract

Abstract: Noxious Chattonella species cause many red tides and fish kills. The detection of extracellular O2 − produced by these algae will be critical for the development of a rapid toxicity-evaluation method; however, such a development has been prevented by the mismatch of the extracellular O2 − detection level between field and laboratory experiments. The aim of this study was to identify impediments to detect extracellular O2 − in laboratory experiments. Our results indicate that Mn and Co in the medium decreased the detection levels of extracellular O2 − produced by Chattonella antiqua, and the effect by Mn was stronger than that by Co. IC50 of the radical-scavenging activity of Mn was 17.5 μM, and little activity was detected at 8.75 μM or less. Furthermore, the level of extracellular O2 − production by C. antiqua in Mn-depleted medium varied greatly under various culture conditions; the extracellular O2 − production level of C. antiqua was high under conditions similar to those under which blooms develop in the field. Therefore, Mn is a key scavenger of extracellular O2 −, and amounts of Mn must be carefully considered whenever extracellular O2 − production levels of phytoplankton cultured with other kinds of media are measured.