High-Throughput-Methyl-Reading (HTMR) assay: a solution based on nucleotide methyl-binding proteins enables large-scale screening for DNA/RNA methyltransferases and demethylases. Issue 2 (29th October 2021)

• Record Type: Journal Article
• Authors: Xiao, Senhao; Guo, Siqi; Han, Jie; Sun, Yanli; Wang, Mingchen; Chen, Yantao; Fang, Xueyu; Yang, Feng; Mu, Yajuan; Zhang, Liang; Ding, Yiluan; Zhang, Naixia; Jiang, Hualiang; Chen, Kaixian; Zhao, Kehao; Luo, Cheng; Chen, Shijie
• Is Part Of: Nucleic acids research. Volume 50:Issue 2(2022)
• Page Start: e9
• Page End: e9
• Publication Date: 2021-10-29
• Subjects: Nucleic acids -- Periodicals; Molecular biology -- Periodicals; 572.805
• DOI: 10.1093/nar/gkab989
• Languages: English
• ISSNs: 0305-1048

Abstract

Abstract: Epigenetic therapy has significant potential for cancer treatment. However, few small potent molecules have been identified against DNA or RNA modification regulatory proteins. Current approaches for activity detection of DNA/RNA methyltransferases and demethylases are time-consuming and labor-intensive, making it difficult to subject them to high-throughput screening. Here, we developed a fluorescence polarization-based 'High-Throughput Methyl Reading' (HTMR) assay to implement large-scale compound screening for DNA/RNA methyltransferases and demethylases-DNMTs, TETs, ALKBH5 and METTL3/METTL14. This assay is simple to perform in a mix-and-read manner by adding the methyl-binding proteins MBD1 or YTHDF1. The proteins can be used to distinguish FAM-labelled substrates or product oligonucleotides with different methylation statuses catalyzed by enzymes. Therefore, the extent of the enzymatic reactions can be coupled with the variation of FP binding signals. Furthermore, this assay can be effectively used to conduct a cofactor competition study. Based on the assay, we identified two natural products as candidate compounds for DNMT1 and ALKBH5. In summary, this study outlines a powerful homogeneous approach for high-throughput screening and evaluating enzymatic activity for DNA/RNA methyltransferases and demethylases that is cheap, easy, quick, and highly sensitive. Graphical Abstract: