Bifunctional Protein Conferring Enhanced Green Fluorescence and Puromycin Resistance. (August 2001)

• Record Type: Journal Article
• Authors: Abbate, J.; Lacayo, J.C.; Prichard, M.; Pari, G.; McVoy, M.A.
• Is Part Of: Biotechniques. Volume 31:Number 2(2001)
• Page Start: 336
• Page End: 340
• Publication Date: 2001-08
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/01312st05
• Languages: English
• ISSNs: 0736-6205

Abstract

A new genetic marker was created in which sequences from enhanced green fluorescent protein were fused to those of puromycin N-acetyl transferase. The resulting fusion protein (EGFP-puro) conferred both green fluorescence and resistance to puromycin when expressed in mammalian cells. The utility of EGFP-puro as a selectable/screenable marker was demonstrated by the ease with which a recombinant guinea pig cytomegalovirus containing EGFP-puro was isolated by a combination of puromycin selection and screening for green fluorescence. We conclude that EGFP-puro is a compact and versatile marker that should prove useful for recombinant virus and transgenic cell line construction, particularly in applications in which coding capacity is limited.