96-Well Plate-Based Method for Total Collagen Analysis of Cell Cultures. (May 2001)

• Record Type: Journal Article
• Authors: Fenwick, S.A.; Curry, V.; Clements, S.; Hazleman, B.L.; Riley, G.P.
• Is Part Of: Biotechniques. Volume 30:Number 5(2001)
• Page Start: 1010
• Page End: 1014
• Publication Date: 2001-05
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/01305st07
• Languages: English
• ISSNs: 0736-6205

Abstract

Total collagen assays are often laborious and use large quantities of consumables. We have developed a new method of assaying total 3 H-proline-labeled collagen from cultured cells. Cells and media are harvested from 96-well plates directly onto fiberglass filtermats and counted in the Wallac 1205 flat-bed scintillation counter (BetaPlate™). The assay was validated by comparison with a traditional total collagen assay. The resulting assay provides a rapid one-step method for quantifying collagen synthesis, which, unlike many collagen assays, does not require extensive dialysis or precipitation of proteins.