Automated Image Analysis of Live/Dead Staining of the Fungus Aureobasidium pullulans on Microscope Slides and Leaf Surfaces. (October 2000)

• Record Type: Journal Article
• Authors: Nelson, Christopher D.; Spear, Russell N.; Andrews, John H.
• Is Part Of: Biotechniques. Volume 29:Number 4(2000)
• Page Start: 874
• Page End: 882
• Publication Date: 2000-10
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/00294rr04
• Languages: English
• ISSNs: 0736-6205

Abstract

An image analysis program and protocol for the identification and enumeration of live versus dead cells of the yeast-like fungus Aureobasidium pullulans was developed for both populations on microscope slides and leaf surfaces. Live cells took up CellTracker Blue™, while nonviable cells stained with DEAD Red™. Image analysis macro programs running under Optimas™ software were used to acquire images and to differentiate and enumerate viable from nonviable cells. The software was capable of discriminating green as a third parameter for identification and quantification of green fluorescent protein-expressing cells in a wild-type population.