A Concise Guide to cDNA Microarray Analysis. (September 2000)

• Record Type: Journal Article
• Authors: Hegde, P.; Qi, R.; Abernathy, K.; Gay, C.; Dharap, S.; Gaspard, R.; Hughes, J.E.; Snesrud, E.; Lee, N.; Quackenbush, J.
• Is Part Of: Biotechniques. Volume 29:Number 3(2000)
• Page Start: 548
• Page End: 562
• Publication Date: 2000-09
• Subjects: Biology, Experimental -- Periodicals; Molecular biology -- Periodicals; Medical technology -- Periodicals; Biology, Experimental; Medical technology; Molecular biology; Clinical Laboratory Techniques -- Periodicals; Research -- Periodicals; Medical Laboratory Science -- Periodicals; Periodicals; Electronic journals; 570
• DOI: 10.2144/00293bi01
• Languages: English
• ISSNs: 0736-6205

Abstract

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.