Ornithinimicrobium laminariae sp. nov., isolated from the kelp Laminaria japonica. Issue 1 (19th January 2022)

• Record Type: Journal Article
• Authors: Cao, Wen-Rui; Yu, Xin-Ran; Sun, Yuan-yuan; Jiang, Ming-Yu; Xu, Xiao-Dong; Li, Ying-Jie
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 1(2022)
• Publication Date: 2022-01-19
• Subjects: Actinobacteria -- Ornithinimicrobiaceae -- Ornithinimicrobium laminariae -- polyphasic taxonomy; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005187
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-positive, aerobic, non-sporulating, yellow-pigmented and rod or cocci-shaped bacterium, designated Arc0846-15 T, was isolated from the kelp Laminaria japonica . Strain Arc0846-15 T was found to grow at 16–35 °C (optimum, 28 °C), at pH 6.0–9.5 (optimum, 7.0) and in the presence of 0–6 % (w/v) NaCl (optimum, 2 %). Cells were positive for catalase and negative for oxidase activity. Phylogenetic analyses, based on 16S rRNA gene sequence comparisons, revealed that the nearest phylogenetic neighbour strains of strain Arc0846-15 T were Ornithinimicrobium murale 01 Gi-040 T (96.2 %), Ornithinimicrobium kibberense K22-20 T (96.1 %) and Ornithinimicrobium humiphilum HKI 0124 T (95.2 %). Based on phylogenomic analysis, the average nucleotide identity values between strain Arc0846-15 T and the neighbour strains were 69.8, 69.7 and 69.8 %, respectively; the digital DNA–DNA hybridization values between strain Arc0846-15 T and its three closest neighbour strains were 18.8, 19.1 and 19.3 %, respectively. The predominant menaquinone was MK-8 (H4 ). The dominant cellular fatty acids were C17 : 1 ω 8 c, iso-C15 : 0, iso-C16 : 0 and C17 : 0 . The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipid, one unidentified aminolipid and four unidentified lipids. The DNA G+C content of strain Arc0846-15 T was 61.6 mol% based on the whole genome sequence. Based on the phylogenetic and phenotypic characteristics, strain Arc0846-15 T is considered to represent a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium laminariae sp. nov. is proposed, with Arc0846-15 T (=KCTC 49655 T =MCCC 1K06093 T ) as the type strain.