Flavobacterium endoglycinae sp. nov., an endophytic bacterium isolated from soybean (Glycine max L. cv. Gwangan) stems. Issue 2 (7th February 2022)

• Record Type: Journal Article
• Authors: Seo, Jiyoon; Peng, Yuxin; Jiang, Lingmin; Lee, Sang-Beom; Jeong, Rae-Dong; Park, Soon Ju; Kim, Cha Young; Choi, Man-Soo; Lee, Jiyoung
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 2(2022)
• Publication Date: 2022-02-07
• Subjects: Flavobacterium endoglycinae sp. nov. -- Glycine max -- novel species; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005220
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : A Gram-stain-negative, facultatively anaerobic, motile by gliding, rod-shaped, oxidase- and catalase-positive bacterial strain, designated BB8 T, was isolated from the stems of a Korean soybean cultivar ( Glycine max L. cv. Gwangan). The strain produced a yellow pigment on tryptic soy agar. Growth of strain BB8 T occurred at pH 5.0–8.0 (optimum, pH 7.0), at 10–35 °C (optimum, 25–30 °C) and in the presence of 0–1 % (w/v) NaCl (optimum, 0.5%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BB8 T formed a lineage within the genus Flavobacterium and was most closely related to Flavobacterium artemisiae SYP-B1015 T (96.9 % 16S rRNA gene sequence similarity) and Flavobacterium ustbae T13 T (96.8%). The complete genome sequence of strain BB8 T was 5 513 159 bp long with a G+C content of 34.1 mol%. The major fatty acids (>10 %) of strain BB8 T were iso-C15 : 0 (21 %), summed feature 3 (comprising C16 : 1 ω7 c and/or C16 : 1 ω6 c, 20.3%) and iso-C16 : 0 3-OH (13.7%). The predominant polar lipids were phosphatidylethanolamine and unidentified aminolipids, and the major respiratory quinone was menaquinone-6. Based on these phenotypic, genotypic and chemotaxonomic characteristics, strain BB8 T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium endoglycinae sp. nov. is proposed. The type strain is BB8 T (=KCTC 82167 T =CCTCC AB 2020070 T ).