A Preliminary Study for an Intraoperative 3D Bioprinting Treatment of Severe Burn Injuries. Issue 1 (31st January 2022)

• Record Type: Journal Article
• Authors: Albouy, Marion; Desanlis, Adeline; Brosset, Sophie; Auxenfans, Celine; Courtial, Edwin-Joffrey; Eli, Kyle; Cambron, Scott; Palmer, Justin; Vidal, Luciano; Thépot, Amélie; Dos Santos, Morgan; Marquette, Christophe A.
• Is Part Of: Plastic and reconstructive surgery. Volume 10:Issue 1(2022)
• Page Start: e4056
• Publication Date: 2022-01-31
• Subjects: Surgery, Plastic -- Periodicals; Surgery, Plastic -- Periodicals; Reconstructive Surgical Procedures -- Periodicals; 617.95205
• DOI: 10.1097/GOX.0000000000004056
• Languages: English
• ISSNs: 2169-7574

Abstract

Abstract : Background: Intraoperative three-dimensional fabrication of living tissues could be the next biomedical revolution in patient treatment. Approach: We developed a surgery-ready robotic three-dimensional bioprinter and demonstrated that a bioprinting procedure using medical grade hydrogel could be performed using a 6-axis robotic arm in vivo for treating burn injuries. Results: We conducted a pilot swine animal study on a deep third-degree severe burn model. We observed that the use of cell-laden bioink as treatment substantially affects skin regeneration, producing in situ fibroblast growth factor and vascular endothelial growth factor, necessary for tissue regeneration and re-epidermalization of the wound. Conclusions: We described an animal study of intraoperative three-dimensional bioprinting living tissue. This emerging technology brings the first proof of in vivo skin printing feasibility using a surgery-ready robotic arm-based bioprinter. Our positive outcome in skin regeneration, joined with this procedure's feasibility, allow us to envision the possibility of using this innovative approach in a human clinical trial in the near future.