Advanced synthesis and application of Nano SiC@ β-glucosidase@ Fe3O4 composite. Issue 3 (March 2021)

• Record Type: Journal Article
• Authors: Deng, Xu; Wu, Xiuwen; Chen, Haonan; Zhang, Yuehang
• Is Part Of: IOP conference series. Volume 692:Issue 3(2021)
• Publication Date: 2021-03
• Subjects: Earth sciences -- Periodicals; Environmental sciences -- Congresses; Environmental sciences -- Periodicals; 550.5
• DOI: 10.1088/1755-1315/692/3/032105
• Languages: English
• ISSNs: 1755-1307

Abstract

Abstract: An improved method for immobilizing β-glucosidase was studied in present work. The immobilization included the following steps: preparing a nano silicon carbi de carrier, mixing the carrier, β-glucosidase and a cross-linking agent to obtain immob ilized β-glucosidase. Then, the immobilized β-glucosidase was mixed with Fe3 O4 nano particles and self-assembled to obtain nano-silicon carbide@β-glucosidase@ Fe3 O4 co mposite. This method improved the activity of immobilized β-glucosidase, and solved the technical problems of low β-glucosidase reuse rate and low enzyme activity retenti on rate.