ALA‐PDT alleviates the psoriasis by inhibiting JAK signalling pathway. Issue 11 (23rd October 2019)

• Record Type: Journal Article
• Authors: Yi, Fei; Zheng, Xiaoli; Fang, Fang; Zhang, Jiaan; Zhou, Bingrong; Chen, Xiangsheng
• Is Part Of: Experimental dermatology. Volume 28:Issue 11(2019)
• Page Start: 1227
• Page End: 1236
• Publication Date: 2019-10-23
• Subjects: JAK pathway -- keratinocytes -- Photodynamic therapy -- reactive oxygen species; Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.14017
• Languages: English
• ISSNs: 0906-6705

Abstract

Abstract: Background: Photodynamic therapy (PDT) with 5‐aminolevulinic acid (ALA) is a well‐known treatment for non‐hypertrophic actinic keratoses and superficial basal‐cell carcinomas. Objectives: In this study, we first revealed that ALA‐PDT treatment effectively ameliorated the psoriasis‐like lesion in imiquimod (IMQ)‐induced mouse model and further explored the potential molecular mechanism and related signalling pathways during the treatment. Besides, we also confirmed a significant alleviation of ALA‐PDT therapy on IFN‐γ‐induced over‐proliferation of keratinocytes. Methods: H&E staining was conducted to reveal the histological changes of mice in different groups. The different expression levels of RNA were illustrated by using QRT‐PCR. Western blot was performed to confirm the various expression levels of protein in mice. In vitro, cell proliferation and cell cycle were evaluated by cell counting kit‐8 and flow cytometry assay, respectively. Results: The result showed that ALA‐PDT's anti‐proliferation effect and regulation on Socs1 /3, JAK1 /2 and K17 in IFN‐γ‐induced keratinocytes were largely weakened by NAC, indicating that ALA‐PDT attenuated the proliferation of IFN‐γ‐induced keratinocytes by enhancing ROS level. Conclusions: These results demonstrated that ALA‐PDT largely activated the productivity of Socs1 /3 in a ROS‐dependent manner. Socs1 /3 is a potential blocker in JAK signalling pathway and inhibits the proliferation and keratinization of keratinocytes in psoriasis.