Mechanical writing of electrical polarization in poly (L-lactic) acid. (February 2022)

• Record Type: Journal Article
• Authors: Barroca, Nathalie; Collins, Liam; Rodriguez, Brian J.; Fernandes, M.Helena V.; Vilarinho, Paula M.
• Is Part Of: Acta biomaterialia. Volume 139(2022)
• Page Start: 249
• Page End: 258
• Publication Date: 2022-02
• Subjects: Poly (L-lactic) acid -- Mechanical writing -- Electrical polarization -- Kelvin probe force microscopy; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2021.05.057
• Languages: English
• ISSNs: 1742-7061

Abstract

Abstract: Stimuli responsive materials are found in a broad range of applications, from energy harvesters to biomolecular sensors. Here, we report the production of poly (L-lactic acid) (PLLA) thin films that exhibit a mechanical stress responsive behaviour. By simply applying a mechanical stress through an AFM tip, a local electrical polarization was generated and measured by Kelvin Probe Force Microscopy. We showed that the magnitude of the stress generated electrical polarization can be manipulated by varying the thickness or crystallization state of the PLLA thin films. Besides exhibiting a mechanical stress-response behaviour with potential for energy harvesting and sensor applications, we show by AFM that these platforms react to mechanical forces with physiological relevance: interaction forces as low as a cell sheet migrating over a substrate or larger ones as the fluid induced stresses in bone tissue. In living tissues, as most mechanical stimuli are transduced as strain gradients for the anatomical structures, these mechanically responsive substrates can be used as ex vivo platforms to study the protein and cells response over a large range of electrical stimuli amplitude. As a proof of concept, selective adsorption of a human fibronectin was demonstrated by local patterning of the stimuli responsive PLLA films. Statement of significance: Bioelectricity is inherent to the formation and repair of living tissues and electrical stimulation has been recognized for promoting regeneration. Given the proven beneficial effects of electric fields and the absence of a suitable method of stimulation, there is a clinical need for smart substrates, which can generate a polarization (charges) to promote tissue regeneration without the need of external devices. In this work, we report the fabrication of poly(L-lactic) acid platforms that exhibit a mechanical stress responsive behaviour when subjected to physiologically relevant forces. This behaviour can be tailored by varying the thickness or crystallization state of the PLLA films. We further demonstrate the biofunctionality of such platforms by exploiting the mechanically-induced charge for adhesion protein adsorption. Graphical abstract: Image, graphical abstract