Biomass purge strategies to control the bacterial community and reactor stability for biohydrogen production from winery wastewater. (29th January 2022)

• Record Type: Journal Article
• Authors: Mejía-Saucedo, Carolina; Buitrón, Germán; León-Galván, Ma. Fabiola; Carrillo-Reyes, Julián
• Is Part Of: International journal of hydrogen energy. Volume 47:Number 9(2022)
• Page Start: 5891
• Page End: 5900
• Publication Date: 2022-01-29
• Subjects: Agro-industrial effluent -- Dark fermentation -- Lactate fermentation -- Structured bed reactor; Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2021.12.007
• Languages: English
• ISSNs: 0360-3199

Abstract

Abstract: Hydrogen production is a viable alternative for the valorization of agro-industrial effluents, such as winery wastewater (WW). One limitation of using new substrates is system stability, where high organic loading rates favor high productivities. Using a new reactor configuration of packed bed reactors, different biomass purge strategies were evaluated in parallel reactors to maintain the stability in the long-term feeding WW, along with a community evaluation. A frequently programmed purge of biofilm and suspended biomass (every 7–8 days) resulted in the most stable hydrogen productivity, 930 mL H2 L −1 d −1, unlike only purging biofilm or not programing the purge (<650H2 L −1 d −1 ). The long-term stability is explained by a proper balance of the microbial genera in the reactor, Lactobacillus, Pectinatus, and Clostridium . This work proposes an innovative reactor configuration and operation strategy using WW, where a programmed biomass purge helps control the lactate and hydrogen-producing microbial groups. Highlights: Winery wastewater is a potential substrate for biohydrogen production. In biofilm reactors, a frequent purge ensure the long term stability. Biofilm and suspended biomass withdrawn is the most suitable purge strategy. The biomass purge selected a balanced microbial community. Lactate consumption is assumed as the leading hydrogen-producing pathway.