Sustainable and optimized bioethanol production using mix microbial consortium of Saccharomyces cerevisiae and Candida cantarelli. (15th April 2022)

• Record Type: Journal Article
• Authors: Kamal, Shagufta; Rehman, Saima; Rehman, Kanwal; Ghaffar, Abdul; Bibi, Ismat; Ahmed, Tanvir; Maqsood, Sumbel; Nazish, Nadia; Iqbal, Hafiz M.N.
• Is Part Of: Fuel. Volume 314(2022)
• Publication Date: 2022-04-15
• Subjects: Bioethanol -- Agro-industrial wastes -- Corn stover -- Consortium -- Box Behnken Design -- Regression analysis; Fuel -- Periodicals; Coal -- Periodicals; Coal; Fuel; Periodicals; 662.6
• DOI: 10.1016/j.fuel.2021.122763
• Languages: English
• ISSNs: 0016-2361

Abstract

Graphical abstract: Highlights: Herein, sustainable transformation of agro-waste into high-value fuel is reported. Bioethanol was produced using mix microbial consortium of S. cerevisiae and C. cantarelli . Three factor Box Behnken Design and regression analysis was employed for optimum ethanol yield. Up to 92.5 g/L ethanol yield was obtained after 6 h fermentation using mix microbial consortium. Abstract: Agro-industrial wastes, the most abundant, readily available, and economical materials are of supreme interest that supports sustainable transformation into high-value fuels. However, the low productivity of ethanol due to the inhibition from the degradation product is a significant concern. In the present study, this limitation has been overcome by fermentation of pre-treated saccharified corn stover (CS) with co-cultures of locally isolated and identified strains, i.e., Saccharomyces cerevisiae (FBL-01) and Candida cantarelli (FBL-01). Three factor Box Behnken Design (BBD) and regression analysis was employed for optimum yield of ethanol. The lignin content in residual solids was decreased to 3.95%, although 91.28% of lignin was decomposed in CS after pre-treatment with 1% (w/v) NaOH. HPLC analysis revealed that concentration of reducing sugars; mannose 21.7 g, fructose 15.08 g, glucose 24.5 g were present in saccharified CS whereas activities of β-glucosidase, endo -glucanase, and exo -glucanase were 46.8 ± 1.43, 53.5 ± 1.24 and 41.3 ± 1.31 U/mL, respectively. SEM analysis confirmed that every step, i.e., pre-treatment, saccharification, fermentation decreased crystallinity, and degree of polymerization of CS. After 6 h fermentation using 3:2 mL ( S . cerevisae : C . cantarelli ) inoculum size ratio at pH 6 and 35 °C, up to 92.5 g/L ethanol yield was obtained. The present study suggested that bioethanol production could be effectively enhanced by co-cultures of yeast using BBD from corn stover.