Biocomponents from Opuntia robusta and Opuntia streptacantha fruits protect against diclofenac-induced acute liver damage in vivo and in vitro. (February 2022)

• Record Type: Journal Article
• Authors: Villa-Jaimes, Gloria Stephanie; Aguilar-Mora, Fabio Alejandro; González-Ponce, Herson Antonio; Avelar-González, Francisco Javier; Martínez Saldaña, Ma. Consolación; Buist-Homan, Manon; Moshage, Han
• Is Part Of: Journal of functional foods. Volume 89(2022)
• Publication Date: 2022-02
• Subjects: Diclofenac -- Hepatocyte -- Apoptosis -- Natural products -- Antioxidants; ADR adverse drug reaction -- ATP adenosine triphosphate -- AUF arbitrary units of fluorescence -- Bet betanin -- Bet+DF betanin plus diclofenac -- CNG cyclic nucleotide-gated -- CTCF corrected total cell fluorescence -- DF diclofenac -- DILI drug-induced liver injury -- DISC death-inducing signaling complex -- FIC fold induction vs. control -- GSH glutathione -- GSSG glutathione disulfide -- H2O2 hydrogen peroxide -- HO-1 heme oxygenase-1 -- JNK c-Jun N-terminal kinase -- NAC N-acetylcysteine -- NAC+DF N-acetylcysteine plus diclofenac -- OR Opuntia robusta -- OR+DF O. robusta plus diclofenac -- OS Opuntia streptachantha -- OS+DF O. streptacantha plus diclofenac -- OTC over the counter -- PKA protein kinase A -- MGV mean gray value -- MMP mitochondrial membrane polarization -- MPTP permeability transition pore -- NSAID non-steroidal anti-inflammatory drug -- ROS reactive oxygen species -- RNS reactive nitrogen species -- SOD Superoxide dismutase -- 4-OH 4′-hydroxydiclofenac -- 5-OH 5′-hydroxydiclofenac; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2022.104960
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Diclofenac induces acute-liver damage causing oxidative stress by mitochondrial dysfunction. Opuntia extracts prophylactically protect against diclofenac-induced acute liver toxicity. The protective effect of Opuntia extracts is related to their antioxidant properties to prevent cell dysfunction and death. Antioxidant activity of Opuntia extracts is due to their biocomponents content such as betacyanins. Abstract: This study aimed to investigate whether Opuntia spp- extracts protect against diclofenac (DF)-induced hepatotoxicity. Rats were pretreated with Opuntia extracts, betanin (Bet) and N-acetylcysteine (NAC) followed by a single challenge of diclofenac. Liver tissue was collected for biochemical and histological analysis. Primary rat hepatocytes were treated with diclofenac (400 µmol/L) with and without pretreatment with Opuntia extract. Apoptosis was measured by caspase-3 activity and necrosis by Sytox green staining. RNA was isolated, and real-time qPCR was performed to assess mRNA levels of stress and apoptosis-related genes MnSOD (SOD2), GADD45B and P53. ROS production was measured using the fluorescent MitoSOX assay. Results demonstrated that Opuntia spp -extracts protect against DF-induced liver toxicity via reducing oxidative stress and the inhibition of P53.