Start-up of mainstream anammox process through inoculating nitrification sludge and anammox biofilm: Shift in nitrogen transformation and microorganisms. (March 2022)

• Record Type: Journal Article
• Authors: Zhang, Wenke; Yu, Deshuang; Zhang, Jianhua; Miao, Yuanyuan; Zhao, Xinchao; Ma, Guocheng; Li, Jiawen; Zhang, Yu
• Is Part Of: Bioresource technology. Volume 347(2022)
• Publication Date: 2022-03
• Subjects: Mainstream single-stage partial nitrification-anammox -- Start-up -- Intermittent aeration -- Integrated fixed-film activated sludge -- Evolution of microbial community; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.126728
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: SPNA-IFAS reactor was started up by seeding nitrification sludge and anammox biofilm. NOB migrated from flocs to biofilm while AnAOB enriched in flocs when SRT decreased. Short SRT of flocs may play a limited role in inhibiting NOB. Short SRT of flocs may decrease AnAOB abundance and deteriorate nitrogen removal. Simultaneous fermentation, partial denitrification-anammox was speculated in biofilm. Abstract: The feasibility of starting up mainstream single-stage partial nitrification-anammox (SPNA) system by inoculating nitrification sludge and anammox biofilm was investigated. The SPNA system treating low-strength synthetic wastewater was rapidly started up with TN removal efficiency of 88.5 ± 1.8% and effluent nitrate concentration of 7.2 ± 1.2 mg/L. Both the abundance and maximum activity of nitrite oxidizing bacteria (NOB) in flocs decreased obviously. Interestingly, the abundance of anaerobic ammonium oxidizing bacteria (AnAOB) in flocs increased from 0.213% to 0.346% despite the sludge retention time (SRT) of flocs decreased to 60 days, the AnAOB in biofilm was 0.434%. That meant AnAOB gradually enriched in flocs and accounted for a fairly high proportion. The inhibition of NOB, partial denitrification and increased aerobic_chemoheterotrophy function in flocs might be the main reasons for AnAOB enrichment. The possibility of simultaneous fermentation, partial denitrification and anammox reaction was predicted in biofilm, further improving the stability of the system.