Synergistic activity and immunomodulatory potential of levofloxacin and Synoeca‐MP peptide against multi-resistant strains of Klebsiella pneumoniae. (February 2022)

• Record Type: Journal Article
• Authors: Ormondes de Farias, Jade; Resende Ferreira, Arthur Corrêa; Cardoso Kostopoulos, Alessandra Godoi; Berto Rezende, Taia Maria; Dias, Simoni Campos
• Is Part Of: Microbial pathogenesis. Volume 163(2022)
• Publication Date: 2022-02
• Subjects: Synoeca-MP -- Antimicrobial peptide -- Klebsiella pneumoniae -- Immune system -- Inflammatory mediators; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2022.105403
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: The purpose of this article is to study the isolated and combined effect of the peptides Synoeca-MP and IDR-1018 against multi-resistant clinical isolates of K. pneumoniae (Kp2177569 - LACEN) in vitro . The bactericidal activity of the peptide Synoeca-MP in combination with three different classes of commercial antimicrobials and its immunomodulatory potential was also evaluated. Synoeca-MP showed better antimicrobial activity than IDR-1018 and presented synergistic action combined with levofloxacin. Therefore, Synoeca-MP and levofloxacin, and the combination of both, were used in subsequent analyses. In the presence of heat-killed antigens, cellular viability and TNF-α levels was maintained, the production of NO increased and a reduction in IL-10 production was observed. The synergistic antibacterial effect between Synoeca-MP and levofloxacin was effective against multidrug-resistant strains of K. pneumoniae . The association of Synoeca-MP and levofloxacin may present a low modulating action of pro and anti-inflammatory mediators, based on these results. Highlights: Synoeca-MP has better antimicrobial activity than IDR-1018 and shows synergistic action in combination with levofloxacin. Synoeca-MP and levofloxacin combination was not cytotoxic to RAW 264.7 cell culture. Synoeca-MP and levofloxacin combination did not increase nitric oxide production in RAW 264.7 cell culture. Synoeca-MP and levofloxacin combination did not alter TNF-α production in RAW 264.7 cell culture. Synoeca-MP and levofloxacin combination decreased levels of IL-10 in RAW 264.7 cell culture.