Efficient saccharification of wheat straw pretreated by solid particle-assisted ball milling with waste washing liquor recycling. (March 2022)

• Record Type: Journal Article
• Authors: Liu, Xiao-le; Dong, Chengyu; Leu, Shao-Yuan; Fang, Zhen; Miao, Zheng-diao
• Is Part Of: Bioresource technology. Volume 347(2022)
• Publication Date: 2022-03
• Subjects: Pretreatment -- Solid particle -- Ball milling -- Enzymatic hydrolysis -- Wheat straw; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2022.126721
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Wheat straw was ball milling pretreated with NaOH particles for enzymatic hydrolysis. Highlights: NaOH particles added during BM showed the best synergistic interaction effect. NaOH-BM treatment decreased CrI and D50 by 1.1 and 6.9 - fold in 1 h, respectively. Reducing-end concentration of cellulose increased by 3.8 times from 12.5 - 60.2 μM. Glucose yield (82.4%) with 4 h NaOH BM treatment was 3.1 - fold that of raw straw. Washing liquor was recycled for the re-treatment, reaching 99.4% glucose yield. Abstract: Wheat straw was pretreated using ball milling (BM) promoted by solid particles (NaOH, NaCl, citric acid). NaOH showed the best synergistic interaction effect, due to the breakage of β-1, 4-glycosidic bonds among cellulose molecules by the alkali solid particles induced by BM. NaOH-BM pretreatment decreased the straw crystallinity from 46% to 21.4% and its average particle size from 398.3 to 50.6 μm in 1 h. After 4 h milling, the reducing-end concentration of cellulose increased by 3.8 times from 12.5 to 60.2 μM, with glucose yield increased by 2.1 times from 26.6% to 82.4% for 72 h enzymatic hydrolysis at cellulase loading of 15 FPU/g dry substrate. The pretreatment washing liquor was recycled for the re-treatment of partially pretreated biomass at 121 °C for 30 min, resulting in 99.4% glucose yield by enzymatic hydrolysis. BM assisted with alkali particles was an effective approach for improving biomass enzymatic saccharification.