Evaluation of a cuticle protein gene as a potential RNAi target in aphids. Issue 1 (8th October 2019)

• Record Type: Journal Article
• Authors: Shang, Feng; Ding, Bi‐Yue; Ye, Chao; Yang, Li; Chang, Teng‐Yu; Xie, Jiaqin; Tang, Liang‐De; Niu, Jinzhi; Wang, Jin‐Jun
• Is Part Of: Pest management science. Volume 76:Issue 1(2020)
• Page Start: 134
• Page End: 140
• Publication Date: 2019-10-08
• Subjects: cuticle protein -- RNA interference -- mortality -- aphids -- predator -- biological control; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.5599
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: RNA interference (RNAi) has potential as a pest insect control technique. One possible RNAi target is the cuticle protein, which is important in insect molting and development. As an example, here we evaluate the possibility of designing double‐stranded RNA (RNA) that is effective for silencing the cuticle protein 19 gene ( CP19 ) in aphids but is harmless to non‐target predator insects. RESULTS: The sequences of CP19s were similar (86.6–94.4%) among the tested aphid species ( Aphis citricidus, Acyrthosiphon pisum, and Myzus persicae ) but different in the predator Propylaea japonica . Ingestion of species‐specific dsRNAs of CP19 by the three aphids produced 39.3–64.2% gene silencing and 45.8–55.8% mortality. Ingestion of non‐species‐specific dsRNA (ds AcCP19 ) by Ac. pisum and M. persicae gave gene silencing levels ranging from 40.4% to 50.3% and 43.3–50.8% mortality. The ds ApCP19 did not affect PjCP19 expression or developmental duration in P. japonica . CONCLUSION: The results demonstrate that CP19 is a promising RNAi target for aphid control via one dsRNA design. The targeting of genes that are conserved in insect pests but not present in beneficial insects is a useful RNAi‐based pest control strategy. © 2019 Society of Chemical Industry Abstract : This article reported that ingestion of both species‐specific and non‐species‐specific dsRNA of a conserved cuticle protein ( CP19 ) induced significant mortalities in three aphid species but not in the predator.