Effect of biomolecules derived from human platelet-rich plasma on the ex vivo expansion of human adipose-derived mesenchymal stem cells for clinical applications. (January 2022)

• Record Type: Journal Article
• Authors: Becerra-Bayona, Silvia M.; Solarte, Víctor Alfonso; Alviar Rueda, Juan David; Sossa, Claudia L.; Arango-Rodríguez, Martha L.
• Is Part Of: Biologicals. Volume 75(2022)
• Page Start: 37
• Page End: 48
• Publication Date: 2022-01
• Subjects: Fetal bovine serum FBS -- Human derivatives from platelet-rich plasma hD-PRP -- human adipose-derived MSCs hAD-MSCs -- media supplemented with hD-PRP hD-PRP-SM -- media supplemented with FBS FBS-SM -- Mesenchymal stem cells MSCs -- Bone marrow BM -- Adipose tissue AD -- umbilical cord blood UCB -- Human AD-MSC hAD-MSC -- Citrate phosphate dextose adenine 1 CPDA-1 -- Good manufacturing practice GMP -- Stromal vascular cells SVF -- Room temperature RT -- Phosphate buffer solution PBS -- Cumulative population doubling level CPDL -- Population doubling PD -- Standar deviation SD -- Xenogeneic serum free medium XFM -- Society for Cell Tharapy ISCT -- Peroxisome Proliferator-Activated Receptor PPAR -- Adipocyte Protein 2 aP2 -- Mitogen activated protein kinase MAPK -- Extracellular signal-regulated kinase ERK -- c-Jun N-terminal kinase JNK; Human adipose-derived mesenchymal stem cell -- Fetal bovine serum -- Human derivatives from platelet-rich plasma -- Clinical applications; Biological products -- Standards -- Periodicals; Biological Products -- Periodicals; Biological Products -- standards -- Periodicals; Produits biologiques -- Normes -- Périodiques; Biological products -- Standards; Periodicals; 615.37
• DOI: 10.1016/j.biologicals.2021.11.001
• Languages: English
• ISSNs: 1045-1056

Abstract

Abstract: Mesenchymal stem cells are a tool in cell therapies but demand a large cell number per treatment, for that, suitable culture media is required which contains fetal bovine serum (FBS). However, for cell-based therapy applications, the use of FBS is problematic. Several alternatives to FBS have been explored, including human derivatives from platelet-rich plasma (hD-PRP). Although various studies have evaluated the impact of hD-PRP on MSC proliferation and differentiation, few of them have assessed their influence on processes, such as metabolism and gene expression. Here, we cultured human adipose-derived MSCs (hAD-MSCs) in media supplemented with either 10% hD-PRP (hD-PRP-SM) or 10% FBS (FBS-SM) in order to characterize them and evaluate the effect of hD-PRP on cell metabolism, gene expression of associated regenerative factors, as well as chromosome stability during cell expansion. We found that hAD-MSCs cultured in hD-PRP-SM have a greater cell elongation but express similar surface markers; in addition, hD-PRP-SM promoted a significant osteogenic differentiation in the absence of differentiation medium and increased the growth rate, maintaining chromosomal stability. In terms of cell metabolic profile, hAD-MSC behavior did not reveal any differences between both culture conditions. Conversely, significant differences in collagen I and angiopoietin 2 expression were observed between both conditions. The present results suggest that hD-PRP may influence hAD-MSC behavior. Highlights: Human derivatives from platelet-rich plasma is an alternatives to fetal bovine serum. Human derivatives from platelet-rich plasma promoted perse a significant osteogenic differentiation. Human derivatives from platelet-rich plasma increase the growth rate. Human derivatives from platelet-rich plasma may influence mesenchymal stem cells behavior.