Thermal plasticity of the circadian clock is under nuclear and cytoplasmic control in wild barley. (30th July 2019)

• Record Type: Journal Article
• Authors: Bdolach, Eyal; Prusty, Manas Ranjan; Faigenboim‐Doron, Adi; Filichkin, Tanya; Helgerson, Laura; Schmid, Karl J.; Greiner, Stephan; Fridman, Eyal
• Is Part Of: Plant, cell and environment. Volume 42:Number 11(2019)
• Page Start: 3105
• Page End: 3120
• Publication Date: 2019-07-30
• Subjects: adaptation -- circadian clock -- plasmotype -- plasticity -- QTL by environment interactions -- robustness -- wild barley; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.13606
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Temperature compensation, expressed as the ability to maintain clock characteristics (mainly period) in face of temperature changes, that is, robustness, is considered a key feature of circadian clock systems. In this study, we explore the genetic basis for lack of robustness, that is, plasticity, of circadian clock as reflected by photosynthesis rhythmicity. The clock rhythmicity of a new wild barley reciprocal doubled haploid population was analysed with a high temporal resolution of pulsed amplitude modulation of chlorophyll fluorescence under optimal (22°C) and high (32°C) temperature. This comparison between two environments pointed to the prevalence of clock acceleration under heat. Genotyping by sequencing of doubled haploid lines indicated a rich recombination landscape with minor fixation (less than 8%) for one of the parental alleles. Quantitative genetic analysis included genotype by environment interactions and binary‐threshold models. Variation in the circadian rhythm plasticity phenotypes, expressed as change (delta) of period and amplitude under two temperatures, was associated with maternal organelle genome (the plasmotype), as well as with several nuclear loci. This first reported rhythmicity driven by nuclear loci and plasmotype with few identified variants, paves the way for studying impact of cytonuclear variations on clock robustness and on plant adaptation to changing environments. Abstract : Robustness of circadian rhythms towards high temperature is controlled by nuclear and plasmotype quantitative trait loci in a wild barley ( Hordeum vulgare ssp . spontaneum ) reciprocal doubled haploid population.