Associations between AGT, MTHFR, and VEGF gene polymorphisms and preeclampsia in the Chinese population. (February 2022)

• Record Type: Journal Article
• Authors: Ding, Guifeng; Li, Yan; Gao, Jianrong; Wang, Wenxia; Wang, Huijuan; Bai, Guiqin
• Is Part Of: Placenta. Volume 118(2022)
• Page Start: 38
• Page End: 45
• Publication Date: 2022-02
• Subjects: PE Preeclampsia -- AGT angiotensinogen -- MTHFR methylenetetrahydrofolate reductase -- VEGF vascular endothelial growth factor -- SNP single nucleotide polymorphisms -- RAS Renin-angiotensin system -- MAFs minor allele frequencies -- 95% CI 95%confidence interval -- HWE Hardy - Weinberg equilibrium -- PCR polymerase chain reaction -- OR odds ratio -- BMI body mass index -- LD linkage disequilibrium; Preeclampsia -- AGT -- MTHFR -- VEGF -- Polymorphism; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2022.01.004
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: Introduction: Preeclampsia (PE) is a pregnancy-specific multisystemic syndrome. This study aimed to investigate the associations between angiotensinogen ( AGT ), methylenetetrahydrofolate reductase ( MTHFR ), vascular endothelial growth factor ( VEGF ) polymorphisms, and PE in the Han Chinese population. Methods: We genotyped 26 single-nucleotide polymorphisms (SNP) in three genes by using QuantStudio™ 12 K Flex Real-Time PCR technology in 168 patients with PE and 204 healthy pregnant control subjects. The associations of tested polymorphisms with PE were analyzed at allele, genotype, and haplotype levels. Results: A common coding variant in MTHFR, rs2274976, was significantly associated with increased risk of PE in both allelic and genotype models (P < 0.05). The heterozygous genotypes of rs699 (G/A vs G/G) in AGT gene and rs3025035 (C/T vs C/C) in VEGF gene showed weak associations with increased PE risk, whereas the mutant homozygous genotype of rs3024987 (TT vs C/C) and the heterozygous genotype of rs3025039 (C/T vs C/C) in VEGF gene displayed weak associations with decreased PE risk (P < 0.05). Discussion: However, these weak associations lost significance after multiple testing correction. The results indicated that rs2274976 in MTHFR gene may contribute to the increased risk of PE in pregnant women. AGT and VEGF gene polymorphisms may not play a significant role in PE development. Highlights: Rs2274976 in MTHFR gene may contribute to an increased risk of PE in pregnant women. Some SNPs in AGT and VEGF gene polymorphisms showed a weak association with PE. Genetic association needs to be further confirmed in different populations.