Engineering Cupriavidus necator DSM 545 for the one-step conversion of starchy waste into polyhydroxyalkanoates. (March 2022)

• Record Type: Journal Article
• Authors: Brojanigo, Silvia; Gronchi, Nicoletta; Cazzorla, Tiziano; Wong, Tuck Seng; Basaglia, Marina; Favaro, Lorenzo; Casella, Sergio
• Is Part Of: Bioresource technology. Volume 347(2022)
• Publication Date: 2022-03
• Subjects: Consolidated BioProcessing -- Recombinant amylase expression -- Broken rice -- Sweet potato waste -- 3HB; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126383
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Starchy waste are promising polyhydroxyalkanoates (PHAs) carbon sources. Cupriavidus necator DSM 545 was efficiently engineered for amylases secretion. Recombinant C. necator DSM 545 #11 showed high hydrolytic activity on starch. The recombinant strain produced high PHAs titers from raw corn starch. C. necator DSM 545 #11 confirmed high PHAs titers also on starchy waste. Abstract: Starch-rich by-products could be efficiently exploited for polyhydroxyalkanoates (PHAs) production. Unfortunately, Cupriavidus necator DSM 545, one of the most efficient PHAs producers, is not able to grow on starch. In this study, a recombinant amylolytic strain of C. necator DSM 545 was developed for the one-step PHAs production from starchy residues, such as broken rice and purple sweet potato waste. The glucodextranase G1d from Arthrobacter globiformis I42 and the α-amylase amyZ from Zunongwangia profunda SM-A87 were co-expressed into C. necator DSM 545. The recombinant C. necator DSM 545 #11, selected for its promising hydrolytic activity, produced high biomass levels with noteworthy PHAs titers: 5.78 and 3.65 g/L from broken rice and purple sweet potato waste, respectively. This is the first report on the engineering of C. necator DSM 545 for efficient amylase production and paves the way to the one-step conversion of starchy waste into PHAs.