Ear reconstruction research using animal models: The effect of fat grafting on costal cartilage stents. (February 2022)

• Record Type: Journal Article
• Authors: Chen, Xia; Zhang, Ruhong; Li, Datao; Zhang, Qun; Xu, Zhicheng; Xu, Feng; Li, Yiyuan; Li, Tianya
• Is Part Of: International journal of pediatric otorhinolaryngology. Volume 153(2022)
• Publication Date: 2022-02
• Subjects: Microtia -- Mastoid depression -- Fat graft -- Chondrocytes -- Cartilage; Otolaryngology -- Periodicals; Pediatrics -- Periodicals; Otolaryngology -- Periodicals; Pediatrics -- Periodicals; Oto-rhino-laryngologie -- Périodiques; Pédiatrie -- Périodiques; 618.9209751
• DOI: 10.1016/j.ijporl.2021.111016
• Languages: English
• ISSNs: 0165-5876

Abstract

Abstract: Object: For congenital microtia patients with a depressed mastoid area, it is unclear whether autologous fat grafting to fill the depressed area of the cheek will affect the survival of the subsequent grafted costal cartilage stent. An animal model was used for in vivo research to provide guidance for clinical applications. Methods: Autologous costal cartilage was implanted in nude mice. Fat samples were collected at different time points and histological examination performed to analyze the activity of chondrocytes and the deposition of the chondrocyte matrix. Results: This nude mouse fat transplantation model study showed that there were statistical differences in chondrocyte viability between the fat filling group and the control group, but there was no statistical difference in the effect on collagen content. Conclusion: Transplanting fat reduces the viability of chondrocytes, but has little effect on collagen matrix deposition.