Efficient Microcystis removal and sulfonamide-resistance gene propagation mitigation by constructed wetlands and functional genes analysis. (April 2022)

• Record Type: Journal Article
• Authors: Wang, Bo; Peng, Qin; Wang, Rui; Yu, Shuhua; Li, Qi; Huang, Chunping
• Is Part Of: Chemosphere. Volume 292(2022)
• Publication Date: 2022-04
• Subjects: Metagenomic sequencing -- mlrA gene -- MC-LR -- ARG -- Microbial function prediction -- N-cycling gene; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2021.133481
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Increasingly prevalent Microcystis blooms and the propagation of the associated resistance genes represent global environmental problems. Constructed wetlands (CWs) are a cost-effective technology used for wastewater treatment. In this study, the herb Alisma orientale and three industrial byproducts, namely, blast furnace slag, biochar, and sawdust, were selected to construct mini-CW units. Their potential to remediate toxic Microcystis and their influences on the behaviors of antibiotic-resistant genes (ARGs, sul1, sul2, and intl1 ) were analyzed. Approximately 98.46% of Microcystis cells were removed by the sawdust-based CW in just 2 d, wherein <0.37 μg/L residual microcystin (MC)-LR was detected, with a removal efficiency of >96.47%, which is potentially caused by the higher relative abundance of MC-degrading gene mlr A on the substrate. Lower target ARG accumulations in the sawdust-based CW may be attributed to the lower intl1 relative abundance and microbial function mobile element content, which could influence horizontal gene transfer. In three sequential batches for the treatment of eutrophic lake water, six sawdust-based CW units were assembled into CW microcosms. The efficiency of removal of Microcystis and MC-LR by planted CW microcosms ranged between 92.00% and 95.88% and between 86.48% and 94.82%, respectively; this was significantly ( P < 0.05 ) higher than that by unplanted ones. Less accumulation of target ARGs was also observed in planted CWs. Planting considerably improved nitrogen removal, possibly owing to the enrichment of genes involved in the KEGG nitrogen metabolism pathway in the substrate through metagenomic analysis. Graphical abstract: Image 1 Highlights: A. orientale and a sawdust substrate had an optimal toxic Microcystis removal efficiency. The accumulation of mlrA gene is possibly beneficial to the removal of Microcystis and MC-LR. Planting remarkably improved nitrogen removal and N-cycling genes accumulation. The presence of herb A. orientale also mitigated resistance genes transmission.