Cosolvent enhanced lignocellulosic fractionation tailoring lignin chemistry and enhancing lignin bioconversion. (March 2022)

• Record Type: Journal Article
• Authors: Zhao, Zhi-Min; Meng, Xianzhi; Scheidemantle, Brent; Pu, Yunqiao; Liu, Zhi-Hua; Li, Bing-Zhi; Wyman, Charles E.; Cai, Charles M.; Ragauskas, Arthur J.
• Is Part Of: Bioresource technology. Volume 347(2022)
• Publication Date: 2022-03
• Subjects: Bioconversion -- Cosolvent Enhanced Lignocellulosic Fractionation (CELF) -- Rhodococcus opacus PD630 -- Lignin valorization; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.126367
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: CELF lignin facilitates Rhodococcus opacus PD630 growth and lipid accumulation. CELF cleaves β -O-4 and β -5 bonds completely. Increases in carboxylic acid OH group of CELF lignin benefit microbial conversion. CELF lignin is a promising candidate for producing lipid building blocks. Abstract: Cosolvent Enhanced Lignocellulosic Fractionation (CELF) is an emerging solvolysis pretreatment to fractionate lignocellulosic biomass. Herein, the bioconversion performance of CELF lignin was fully evaluated for the first time. Results showed that CELF lignin possessed higher content of carboxylic acid OH, lower molecular weight, and disappeared β -O-4 and β -5 linkages compared to other two technical lignins including a conventional ethanol organosolv lignin (EOL) and a kraft lignin (KL). Rhodococcus opacus PD630 cell count from CELF lignin fermentation reached the highest value of 3.9 × 10 7 CFU/mL, representing a 62.5% and 77.3% improvement over EOL and KL, respectively. Correspondingly, lipid yield reached 143 mg/L from CELF lignin, which was 36.2% and 26.5% higher than from EOL and KL, respectively. Principal component analysis (PCA) revealed that more carboxylic acid groups and lower molecular weight contributed to the enhanced bioconversion performance of CELF lignin. This study demonstrates that CELF lignin is a promising candidate for bioconversion.