Biosynthesis of fuel-grade ethanol from cellobiose by a cell-factory of non-GMO Saccharomyces cerevisiae/starch-gel-cellulase. (1st April 2022)

• Record Type: Journal Article
• Authors: Kalogeropoulou, Archontoula; Plioni, Iris; Dimitrellou, Dimitra; Soupioni, Magdalini; Nigam, Poonam Singh; Kanellaki, Maria; Koutinas, Athnasios A.
• Is Part Of: Fuel. Volume 313(2022)
• Publication Date: 2022-04-01
• Subjects: OSHF one-step hydrolysis and fermentation -- GMO genetically modified organism -- CF cell factory -- SG starch gel -- ALG alginate -- PEG polyethylene glycol; Cell-factory -- Saccharomyces cerevisiae -- Cellobiose -- Cellulase -- Hydrolysis -- Fuel-ethanol; Fuel -- Periodicals; Coal -- Periodicals; Coal; Fuel; Periodicals; 662.6
• DOI: 10.1016/j.fuel.2021.122986
• Languages: English
• ISSNs: 0016-2361

Abstract

Highlights: A new generation cell factory (CF) of non-engineered S. cerevisiae for cellobiose fermentation. The two bioreactors for cellobiose fermentation are substituted with one. The CF design conceptual character due to its application in white biotechnology bioprocessing. CF substitution of S. cerevisiae by a microorganism for production of another chemical using cellobiose. Future investigation to increase yield as increase of cellobiose concentration leads to reduced yield. Abstract: The aim of this work was to ferment cellobiose to fuel-grade ethanol in one-step bioconversion employing a cell-factory (CF) of non-Genetically Modified Organism (non-GMO) Saccharomyces cerevisiae . The proposed technology involved the production of a freeze-dried preparation of CF of non-GMO S. cerevisiae covered by a layer of starch-gel (SG) containing cellulase enzyme. Parameters studied for one-step bioconversion of cellobiose to ethanol included temperature (33–40 °C), cellobiose concentration (70–150 g/L), and cellulase activity (60–150 FPU/g). High conversion (90%) of cellobiose in a 48 h one-step process was achieved using 70 g/L cellobiose, 150 FPU/g at 30 °C, indicating the efficiency of CF. S. cerevisiae in CF were also capable to ferment at 40 °C with the major role of enzyme activity and cellobiose concentration in fermentation. In our studies, the ethanol yield and conversion of cellobiose were found competitive, with other processes reported with S. cerevisiae as GMO and enzyme co-immobilized in alginates beads . Consequently, the results revealed a new concept for bio-ethanol production from cellobiose in one-step simultaneous-hydrolysis-fermentation (OSHF), which could be a precursor study for large-scale cellulose bioconversion for fuel-grade alcohol production.