Improved tagmentation-based whole-genome bisulfite sequencing for input DNA from less than 100 mammalian cells. (February 2015)

• Record Type: Journal Article
• Authors: Lu, Hanlin; Yuan, Zhimei; Tan, Tao; Wang, Junwen; Zhang, Juyong; Luo, Hui-Juan; Xia, Yudong; Ji, Weizhi; Gao, Fei
• Is Part Of: Epigenomics. Volume 7:Number 1(2015)
• Page Start: 47
• Page End: 56
• Publication Date: 2015-02
• Subjects: DNA methylation -- T-WGBS -- Tn5 transposase -- WGBS; Epigenesis -- Periodicals; Genomics -- Periodicals; Chromatin -- Periodicals; DNA -- Periodicals; 572.87
• DOI: 10.2217/epi.14.76
• Languages: English
• ISSNs: 1750-1911

Abstract

Aim: To develop a whole-genome methylation sequencing method that fulfills the needs for studies using ultra-low-input DNA.Materials & methods: The tagmentation-based whole-genome bisulfite sequencing (T-WGBS) technology is modified, enabling stable library construction with complexity from minimally 0.5 ng of initial genomic DNA, which equals less than 100 mammalian cells.Results: We thoroughly assessed the performance of this T-WGBS method by sequencing the methylomes of a rice strain and pre-implantation embryos of rhesus monkey and compare to traditional WGBS approach, thereby demonstrating the efficacy of this new approach.Conclusion: This new approach is highly attractive for the complete methylome analysis of very few cells, for example, mammalian pre-implantation embryos, or tiny human biopsy specimens.