Membrane fixation enhances guided bone regeneration in standardized calvarial defects: A pre‐clinical study. (15th December 2021)

• Record Type: Journal Article
• Authors: An, Yin‐Zhe; Strauss, Franz‐Josef; Park, Jin‐Young; Shen, Yu Qin; Thoma, Daniel Stefan; Lee, Jung‐Seok
• Is Part Of: Journal of clinical periodontology. Volume 49:Number 2(2022)
• Page Start: 177
• Page End: 187
• Publication Date: 2021-12-15
• Subjects: animal model -- bone regeneration -- collagen membrane -- membrane fixation -- osteogenesis; Periodontics -- Periodicals; 617.6
• DOI: 10.1111/jcpe.13583
• Languages: English
• ISSNs: 0303-6979

Abstract

Abstract: Aim: To determine whether collagen membrane (CM) fixation enhances guided bone regeneration in standardized defects. Materials and Methods: Four 8‐mm‐diameter defects were surgically made in eight rabbit calvaria, and randomly allocated into four groups: control (empty), unfixed‐CM, fixed‐CM, and unfixed‐CM with bone graft (BG + CM) (positive control). After 1‐ and 4‐week healing periods, the animals were sacrificed and quantitative reverse transcription polymerase chain reaction, micro‐computed tomography, and histological outcomes were assessed. Results: At week 1, the expression levels of BMP‐2, FGF‐2, VEGF, and osteocalcin were significantly higher in the fixed‐CM group than in the unfixed‐CM and control groups ( p < .05). Conversely, cathepsin‐K was significantly expressed in the unfixed‐CM group. No significant differences in expression markers were observed between the fixed‐CM and BG + CM groups ( p > .05). At week 4, new bone formation was significantly higher in the fixed‐CM group than the unfixed‐CM and control groups ( p < .05), but similar to the BG + CM group ( p > .05). Conclusions: CM fixation enhances the expression of osteogenic factors similar to BG + CM, leading to significantly more new bone formation. This suggests that the osteogenic potential is greater when membranes are fixed, thereby limiting the necessity of membrane‐supporting materials to enhance bone formation.