Ratiometric fluorescence analysis for miR-141 detection with hairpin DNA-templated silver nanoclusters. Issue 2 (14th December 2021) - Liu, Meiqing
Abstract : Hairpin DNA-templated silver nanoclusters enable ratiometric fluorescence analysis for miRNA detection. In the presence of the target miRNA, the green emission of the silver nanoclusters decreases with the red one increasing concurrently. Abstract : MicroRNAs (miRNAs) are important biomarkers for disease diagnosis and prognosis. Accurate and robust detection of miRNAs greatly facilitates the early diagnosis and progress assessment of certain cancers. The current miRNA detection based on the sole fluorescence intensity at a certain emission wavelength is prone to instrumental and environmental interference, reducing the reliability of detection. Here, we innovatively developed hairpin DNA-templated silver nanoclusters (DNA-AgNCs) as a ratiometric fluorescent probe for miR-141 detection. The native DNA-AgNC probe could emit relatively strong green fluorescence ( λ max at 555 nm) and weak red fluorescence ( λ max at 640 nm) upon excitation at 465 nm and 575 nm, respectively. In the presence of the target miR-141, the green emission of the DNA-AgNCs decreased, with the red one increasing concurrently. The ratio of the fluorescence intensity between the red and the green emission was linearly related to the concentration of miR-141, providing a simple approach for a miRNA assay with relatively higher sensitivity and a single nucleotide specificity in miR-141 detection.