Effect of photo-autotrophic cultural conditions on the biomass productivity and composition of Chlorella vulgaris. Issue 2 (25th January 2022)

• Record Type: Journal Article
• Authors: Nordin, Norazela; Yusof, Norjan; Md Nadzir, Syafiqah; Mohd Yusoff, Mohd Zulkhairi; Hassan, Mohd Ali
• Is Part Of: Biofuels. Volume 13:Issue 2(2022)
• Page Start: 149
• Page End: 159
• Publication Date: 2022-01-25
• Subjects: Chlorella vulgaris -- microalgae biomass -- lipid -- carbohydrate -- biofuel feedstock; Biomass energy -- Periodicals; 662.8805
• DOI: 10.1080/17597269.2019.1652787
• Languages: English
• ISSNs: 1759-7269

Abstract

Abstract: The study aims to investigate the effect of photo-autotrophic cultural conditions on the productivity and biomass composition of Chlorella vulgaris . The following five photo-autotrophic cultural conditions were investigated: light intensity (4000, 10, 500, 17, 000, 23, 000, 30, 000 lux), temperature (25, 28, 32, 35, 40 °C), pH (6, 7, 8, 9, 10), CO2 (0.03, 2.5, 5, 7.5, 10%) and NO3 − (0, 250, 500, 750, 1000 mg/L). Results indicate that lipid and protein yields were increased by 3.19 fold and decreased by 1.47 fold, respectively, under NO3 − deficiency (0 mg/L). Meanwhile, carbohydrate yield was increased by 1.39 fold in 5% CO2 . Further cultivation with parameters of the highest biomass productivity (10, 500 lux, 28 °C, pH 8, 5% CO2 and 500 mg/L NO3 − ) achieved the maximum biomass productivity of 0.468 g/L/day. Moreover, cultivation with parameters of the highest lipid yield (23, 500 lux, 40 °C, pH 8, 0.03% CO2 and 0 mg/L NO3 − ) achieved the maximum lipid yield of 43.70%. The major fatty acid methyl ester compositions produced were C20:0 (39.08%) and C16:0 (37.15%), producing biodiesel with high cetane number and oxidative stability. These promising results provide a comprehensive comparison regarding the effect of photo-autotrophic cultural conditions on microalgae biomass and its potential application as a biofuels feedstock.