Mycoplasma miroungirhinis sp. nov. and Mycoplasma miroungigenitalium sp. nov., isolated from northern elephant seals (Mirounga angustirostris), Mycoplasma phocoenae sp. nov., isolated from harbour porpoise (Phocoena phocoena), and Mycoplasma phocoeninasale sp. nov., isolated from harbour porpoise and California sea lions (Zalophus californianus). Issue 1 (24th January 2022)

• Record Type: Journal Article
• Authors: Volokhov, Dmitriy V.; Furtak, Vyacheslav A.; Blom, Jochen; Zagorodnyaya, Tatiana A.; Gao, Yamei; Gulland, Frances M.
• Is Part Of: International journal of systematic and evolutionary microbiology. Volume 72:Issue 1(2022)
• Publication Date: 2022-01-24
• Subjects: Mycoplasma -- phylogeny -- marine mammals -- seals; Microbiology -- Periodicals; Microbiology -- Classification -- Periodicals; 579
• DOI: 10.1099/ijsem.0.005224
• Languages: English
• ISSNs: 1466-5026

Abstract

Abstract : Seven novel independent strains of Mycoplasma species were isolated from northern elephant seals (ES2806-NAS T, ES2806-GEN T, ES3157-GEN-MYC and ES3225-GEN-MYC), a harbour porpoise (C264-GEN T and C264-NAS T ), and a California sea lion (CSL7498). These strains were phenotypically and genetically characterized and compared to the known Mycoplasma species. Four strains (C264-GEN T, C264-NAS T, CSL7498 and ES2806-NAS T ) hydrolysed arginine but not urea and did not produce acid from carbohydrates. Strains ES2806-GEN T, ES3157-GEN-MYC and ES3225-GEN-MYC did not produced acid from carbohydrates and did not hydrolyse arginine or urea; hence, it is assumed that organic acids are used as the energy source for them. All were isolated and propagated in ambient air supplemented with 5±1 % CO2 at +35–37 °C using either SP4 or PPLO medium. Colonies on solid medium showed a typical fried-egg appearance and transmission electron microscopy revealed a typical mycoplasma cellular morphology. The complete genomes were sequenced for all type strains. Average nucleotide and amino acid identity analyses showed that these novel strains were distant from the phylogenetically closely related Mycoplasma species. Based on these data, we propose four novel species of the genus Mycoplasma, for which the name Mycoplasma miroungirhinis sp. nov. is proposed with the type strain ES2806-NAS T (=NCTC 14430 T =DSM 110945 T ), Mycoplasma miroungigenitalium sp. nov. is proposed with the type strain ES2806-GEN T (=NCTC 14429 T =DSM 110944 T ) and representative strains ES3157-GEN-MYC and ES3225-GEN-MYC, Mycoplasma phocoenae sp. nov. is proposed with the type strain C264-GEN T (=NCTC 14344 T =DSM 110687 T ) and Mycoplasma phocoeninasale sp. nov. is proposed with the type strain C264-NAS T (=NCTC 14343 T =DSM 110688 T ) and representative strain CSL7498. The genome G+C contents are 24.06, 30.09, 28.49 and 29.05% and the complete genome sizes are 779 550, 815 486, 693 115, and 776 009 bp for strains ES2806-NAS T, ES2806-GEN T, C264-GEN T and C264-NAS T, respectively.