Zinc regulation of iron uptake and translocation in rice (Oryza sativa L.): Implication from stable iron isotopes and transporter genes. (15th March 2022)

• Record Type: Journal Article
• Authors: Wu, Qiqi; Liu, Chengshuai; Wang, Zhengrong; Gao, Ting; Liu, Yuhui; Xia, Yafei; Yin, Runsheng; Qi, Meng
• Is Part Of: Environmental pollution. Volume 297(2022)
• Publication Date: 2022-03-15
• Subjects: Fe uptake -- Fe isotopes -- Fe transporter genes -- Zn stress; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2022.118818
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Iron (Fe) is an essential nutrient for living organisms and Fe deficiency is a worldwide problem for the health of both rice and humans. Zinc (Zn) contamination in agricultural soils is frequently observed. Here, we studied Fe isotope compositions and transcript levels of Fe transporter genes in rice growing in nutrient solutions having a range of Zn concentrations. Our results show Zn stress reduces Fe uptake by rice and drives its δ 56 Fe value to that of the nutrient solution. These observations can be explained by the weakened Fe(II) uptake through Strategy I but enhanced Fe(III) uptake through Strategy II due to the competition between Zn and Fe(II) combining with OsIRT1 (Fe(II) transporter) in root, which is supported by the downregulated expression of OsIRT1 and upregulated expression of OsYSL15 (Fe(III) transporter). Using a mass balance box model, we also show excess Zn reduces Fe(II) translocation in phloem and its remobilization from senescent leaf, indicating a competition of binding sites on nicotianamine between Zn and Fe(II). This study provides direct evidence that how Zn regulates Fe uptake and translocation in rice and is of practical significance to design strategies to treat Fe deficiency in rice grown in Zn-contaminated soils. Graphical abstract: Image 1 Highlights: Zn stress weakens Fe(II) uptake but enhances Fe(III) uptake. Zn stress restrains upward-translocation and remobilization of Fe(II)-NA. Zn stress alters translocation and isotope fractionation of Fe in rice.